Iciputulwa ca Maka ya Mumabala

Ukulondolola

 

Inshila shakwesha & Ubulondoloshi

 

INSHILA 1 Inshila ya kwesha

1.1 Ukupekanya Amasuko

Wukutemwisha wwa menshi aya kupopomenwa:Pa ntanshi ya kubomfya, umusango wa kusambamo ufwile ukubweseshiwa ku muputule wa muputule ({{0 {

1.2 Ukupekanya Ifyakumwenako

Umulopa wa nama sha nama shonse walipokelelwe, ukuputulwa pa 4000000 pa maminiti 10, kabili icacilapo ukusontelwe(20)} Serem na kabili kuti yasangwa mu cifyalilwa ukwabula ubulwele bwa ku cifuba[3}}.

Nga cakuti ubukulu bwa cilangililo bwakulisha, mu kushininkisha inshita ya kufwailisha iyalinga iya kwesha ilakonkanacitile. Ukusankanya icakumwenako ica mubili ne casuko ca mulimo wa mubili pa 1:1 mu cipe ca kucemena (70 ndiela{{40}}illi-umupashi wa kuceeceetelamo ububifi no kusunga bwino umusambo / hirus}.

1.3 Inshila ya kwesha

①Take the enzyme label Microplate (according to the number of samples, can be disassembled and used several times), set up two wells each for negative and positive controls, add 100ul of the the premixed mixture to the enzyme label Microplate. Gently shake the sample in the well, using the sealing plate film to cover the plate, and incubate at 37℃for 30 amaminitikwete{4}}

‐Pyeni icikope ca kufimbapo, fumyenipo bwino bwino ifya kucita mu cipe, mubikepo 3000μl iya kusamba amenshi ya kusamba ku cishima cimo na cimo, kabili lyene mubike amenshi mu cishima[11 }} Bwekeshapo amatampulo aya pa muulu, kabili mu kupelako suminisheni ukuuma pa pepala lya kusamba ilya busaka ilya kusamba {30}.

③Dukukongama kwa enzyme:Ukufuuta umuti wa enzyme (50×) imiku 50 (ku cakumwenako: 20 enzyli ya enzyme yacilapo ukubika amano+ 980ishi 1, umutengo uwalinga uwafwaikwa upendelwa ukulingana na impendwa ya fipimo fyakwesha), ukusankanya bwino no kubika pa mbali[5 5}} ukubipilwa

‣unsoni 100μl iya Enzyme Ukusuntinkana kuli cila cishima, ukufimba pa mbale, no kufimba pa madigiri 37 pa maminiti 30{3}}

Icipe ca enzyme, umulimo uwalinga wali fye umo wine na pa muulu wa £ {.

Iciputulwa ca 100μl ica muputule wa pa nshi kuli cila cishima, ukufimba pa mbale no kufimba pa maminiti 10 pa madigiri 37 ukufuma ku lubuutokwetemo[3.

Ukulundapo 50μl ukuleka ukwasuka kuli cila cishima, no kwishiba icafumamo mu maminiti 5{2{2}

 

ICIPITA 2 Ubupilibulo bwa Cifumamo .

 

Umutengo wa OD450 uwa cishima cimo na cimo walibelengwa pa96- uwabelenga bwino bwino{2.

2.1 Ukushininkisha

 

Iciputulwa ca OD450 icalinga ica kutungulula bwino cali < £. na OD450m mu kati ka OD450m iya kucenjela kwabipa / ukukonka bwino kwali Ukucilapo ukucila nelyo ukulingana na 3{5}}5, ifyafumamo fya kwesha fyali fya cine. Nga te ifyo, ukufwailisha kulingile ukubwekeshiwapo.

2.2 Inshila ya Kubomfya

Pa cilangililo cimo na cimo, penda PI (Ukucingilila iya nshita) nga fintu cikonkelepo ukubomfya ica kumwenako icalungishiwa ne mibele ya kucenjela:

2.3 Ubupilibulo bwa Cifumamo

Nga cakuti PI < 20%, cipingulwa nga icabipa(11)}}

Ilyo PI > 30%, cilapingulwa nga cisuma[11)}}

Ilyo 20% Ukucepako nelyo ukulingana na PI Ukucepako nelyo ukulingana na 30%, cipingulwa nga ca kuti cashininkishiwa, kabili cilapanda amano ukubwekeshapo ukusalulula no kwesha pa numa ya mulungu umo pa numa ya mulungu umo uwa.

Ama Tags aya Kufina: ifipe fya kucingililamo umubili ku malwele ya ku mubili, ifya kulya fya ku nsengo ifya ku nse ya ng’ombe, ifya kulya ifya nyama, abapanga ifya kufwala, ifya kupangapanga .