Ukufyala no Kupuma Kwakupumikisha Ubulwele bwa Kupuma

Ukulondolola

Abas Als Alsa

Ukubomfya ukwabula ukufwaya: Ukufyala kwa Porc no Kupuusha

Icimonwa ca Virus ica Mushilo ELISA Kit ni pa mulandu wa kuti umuntu ali no bulwele bwa ku PRRSV mu cifuba ca nkumba mu vitro. Ukufwailisha kulabomfiwa ku kupima umuti wa bucingo ukufuma kuli .

ukufyala no kufyala kwa porc na

ubulwele bwa virus ubwa mu mpanga ya nkumba.

Icimonwa: suka

Ifishibilo fyapeelwa:

Inshila shakwesha & Ubulondoloshi

INSHILA 1 Inshila ya kwesha

1.1 Ukupekanya Amasuko

Wukutemwisha wwa menshi aya kupopomenwa:Ilyo bashilabomfya, umuti wa kusamba ufwile ukubweshiwa ku muputule wa kufimbilishishamo (20-25 digiri ) no kukunkunika bwino bwino pa kuti cibe icalinga. Lyena ukufika pa miku 20 iya kusamba iyabikwa pamo na menshi ayasanguluka (ku cakumwenako, 5mll 5ml iya kusamba iyabikwa pamo ilalundwako ku menshi ayasanguluka 95m), ukusankanya bwino, kabili ukusamba kwa menshi ukwatonta kuti kwasungwa pa 2-8 pa nshiku 7.

1.2 Ukupekanya Ifyakumwenako

Umulopa wa nama onse uwa nama walipokelelwe, uwaonawilwe pa 400000000 pa maminiti 10, kabili icacilapo calongene. Iciputulwa ca nshita kuti capangwa na kabili mu cifyalilwa ukwabula ukupokololwa.

1:50 (ukulundapo ica kumwenako ca 37l ica cilangililo ca 147l) no kusankanya bwino bwino.

Ishiba: Ukukongama kwabipa kabili ukwalinga na ko kulekabila ukucepelwa.

1.3 Inshila ya kwesha

Ukusenda enzymen Macymel (ukulingana ne mpendwa ya fimpashanya, kuti kwafumishiwapo no kubomfiwa imiku iingi), ukulundapo 100lli iya mulomo uwaputulwa ku cishibilo ca eccyme, ukubika ifishima fibili cimo cimo ku filangililo fyabipa ne fisuma, no kulundapo 100 iyabipa ku fyabipa na kuli cimo na cimo ica kukongama. Mu kuicefya, fumyeniko ica kumwenako mu cishima, ukubomfya filimu ya mbale ya kufimbapo ku kufimba pa mbale, no kufimba pa madigiri 37 pa maminiti 30.

‐Puleni filimu ya cipe ca kufimbapo, fumyenipo bwino bwino mu cipe, mubikepo 300μl iya kusamba amenshi ya kusamba ku cishima cimo na cimo, kabili lyene mupooseleko amenshi mu cishima. Bwelelenipo pa matampulo ya pa muulu imiku 5, kabili mu kupelako twalani pa pepala ilya busaka.

Ukulundapo 100μl iya Kukonka kwa Enzyme kuli cila cishima, ukufimba pa mbale, no kufimba pa madigiri 37 pa maminiti 45.

Isuleni filimu ya cipepala ca kufimbapo, fumyenipo bwino bwino mu cipe, mubikepo 300μl iya kusamba amenshi ya kusamba ku cishima cimo na cimo, kabili lyene mupooseleko amenshi mu cishima. Bwelelenipo pa matampulo ya pa muulu imiku 5, kabili mu kupelako twalani pa pepala ilya busaka.

․Ambali 100μl iya kupwisha bwino bwino cila cishima, ukufimba pa mbale no kufimba pa maminiti 10 pa cipimo 37 ukufuma ku lubuuto.

Ulupapulo 50μl leka ubwasuko kuli cila cishima, kabili ishibeni icafumamo mu maminiti 5.

ICIPITA 2 Ubupilibulo bwa Cifumamo .

Umutengo wa OD450 uwa cishima cimo na cimo walibelengwa pa kabelenga wa 96--ilyashi ifinono.

2.1 Ukushininkisha

Iciputulwa ca OD450 ica pa kati ica kucenjela icabipa cali .<0.1 and the average OD450nm value of positive control /the average OD450nm value of negative control was ≥3, the experimental results were valid. Otherwise, the test should be repeated.

2.2 Inshila ya Kubomfya

Pa cilangililo cimo na cimo, penda S/P nga fintu cikonkelepo ukubomfya ica kumwenako icalungishiwa ne mibele ya kucenjela:

2.3 Ubupilibulo bwa Cifumamo

Ilyo S/P<0.46, it is judged as negative.

Ilyo S/P Ukucilapo nelyo ukulingana na 0.56, cipingulwa nga cisuma.

Ilyo 0.46 Ukucepako nelyo ukulingana na S/P .<0.56, it is judged as suspicious, and suggest to repeat sampling and test one week later.

Ama Tags aya Kufina: ukufyala no kupuma umulopa wa bulwele bwa ku nsengo, ubwa ku China ubwa kufyalilamo no kupuma ubulwele bwa ku nsengo, abapanga ifya kulya, abapanga ifya kulya, fakitare